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標題:
spectroscopy and riboflavin
發問:
Can someone pls help me with the following questions...we measured riboflavin at diff. wavelengths as well as DNA and nucleiotides. 1-what additional problems are present in obtaining spectrum below 250nm..is it just the fact that below 250 all light is absorbed and hence no light is transmitted meaning no... 顯示更多 Can someone pls help me with the following questions...we measured riboflavin at diff. wavelengths as well as DNA and nucleiotides. 1-what additional problems are present in obtaining spectrum below 250nm..is it just the fact that below 250 all light is absorbed and hence no light is transmitted meaning no absorbance 2-how can UV spectroscopy be used to get more accurate values for protein concs..as it got something to do with using longer wavelengths??
最佳解答:
1. 250 nm is in the UV region of light, and the spectrometer is often unable to detect this region of light and result in appearing having no transmittance. 2. Since most biological macromolecules (protein in your case) do not absorb light in the visible region (~99% transmittance) but only absorb in the UV region, so the concentration can be determined from the corresponding absorbance in the UV region of light.
spectroscopy and riboflavin
發問:
Can someone pls help me with the following questions...we measured riboflavin at diff. wavelengths as well as DNA and nucleiotides. 1-what additional problems are present in obtaining spectrum below 250nm..is it just the fact that below 250 all light is absorbed and hence no light is transmitted meaning no... 顯示更多 Can someone pls help me with the following questions...we measured riboflavin at diff. wavelengths as well as DNA and nucleiotides. 1-what additional problems are present in obtaining spectrum below 250nm..is it just the fact that below 250 all light is absorbed and hence no light is transmitted meaning no absorbance 2-how can UV spectroscopy be used to get more accurate values for protein concs..as it got something to do with using longer wavelengths??
最佳解答:
1. 250 nm is in the UV region of light, and the spectrometer is often unable to detect this region of light and result in appearing having no transmittance. 2. Since most biological macromolecules (protein in your case) do not absorb light in the visible region (~99% transmittance) but only absorb in the UV region, so the concentration can be determined from the corresponding absorbance in the UV region of light.
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